Current miniature microscope technology for recording of network dynamics in freely behaving animals requires the presence of a wired connection to provide power to the microscope and transmit imaging data to a data acquisition device and computer. We have developed a battery-powered miniaturized microscope which uses a data-logging device to recording the imaging data to a microSD card on the microscope itself. We use these microscopes to record from large populations CA1 hippocampal neurons in animals performing social behaviors or running on a 7.6m track. Recordings with the miniaturized microscope in a model of temporal epilepsy show dramatically reduced stability of place cells firing across days. The wire-free microscope will enable recordings on network dynamics in extremely large or complex environments. We have also developed microscopes that integrate imaging with high channel count electrophysiology recordings as well as microscopes specifically made for non-human primates. These tools will greatly expand the capabilities of miniaturized microscope technology and enable experiments not possible in in the past.
Peyman Golshani obtained his BA in English and Molecular and Cell Biology at UC Berkeley. He then went on to obtain his MD/PhD at UC Irvine where he studied the development of the corticothalamic function with Dr. Edward G. Jones. He completed his residency in neurology at UCLA and completed postdoctoral training with Dr. Felix Schweizer and Dr. Guoping Fan studying the role of DNA methylation in cortical development. He has been a faculty member at UCLA where his lab studies how neural network dynamics drive cognition and how these coordinated activity patterns become altered by neurological disease. His group also develops new open-source tools for imaging neural activity patterns in freely behaving animals.
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